《植物生理学报》 2022, 58(3): 533-542

桔梗萜类合成途径PgMCS和PgHDR基因克隆与原核表达分析

李景¹，刘梦丽¹，余函纹¹，周卓¹，常相伟¹，王举涛¹，查良平^1,2,*，桂双英^1,3,4,*

¹安徽中医药大学药学院, 合肥230012；²安徽省中医药科学院中药资源保护与开发研究所, 合肥230012；³安徽省中医药科学院药物制剂研究所, 合肥230012；⁴安徽省教育厅现代药物制剂工程技术研究中心, 合肥230012

通信作者：查良平；E-mail: zlp_ahtcm@126.com；桂双英；E-mail: guishy0520@126.com

摘　要：

关键词：桔梗; MCS; HDR; 基因克隆; 原核表达

收稿：2021-10-18   修定：2021-12-17

资助：国家自然科学基金区域联合基金项目(U21A20406)、安徽省中央引导地方科技发展专项资金(YDZX20183400004233)、中央本级 重大增减支项目(2060302)和2020年安徽高校合作攻关和公共卫生协同创新项目(GXXT-2020-025)。

Gene cloning and prokaryotic expression analysis for the PgMCS and PgHDR genes from the Platycodon grandiflorum terpenoid biosynthesis pathway

LI Jing¹, LIU Mengli¹, YU Hanwen¹, ZHOU Zhuo¹, CHANG Xiangwei¹, CHANG Xiangwei¹, ZHA Liangping^1,2,*, GUI Shuangying^1,3,4,*

¹1School of Pharmacy, Anhui University of Chinese Medicine (AUCM), Hefei 230012, China; ²2Institute of Conservation and Development of Traditional Chinese Medicine Resources, Anhui Academy of Chinese Medicine, Hefei 230012, China; ³Institute of Pharmaceutics, Anhui Academy of Chinese Medicine, Hefei 230012, China; ⁴Engineering Technology Research Center of Modernized Pharmaceutics, Anhui Education Department (AUCM), Hefei 230012, China

Corresponding author: ZHA Liangping; E-mail: zlp_ahtcm@126.com; GUI Shuangying; E-mail: guishy0520@126.com

Abstract:

Key words: Platycodon grandiflorum; MCS; HDR; gene cloning; prokaryotic expression